捻转血矛线虫阿苯达唑敏感株和耐药株比较转录组学分析

doi:10.11843/j.issn.0366-6964.2019.09.024

Abstract

Abstract: To explore the differences in transcriptome levels between Haemonchus contortus albendazole sensitive and resistant strains, and to find out drug resistance-related genes. Samples of susceptible and resistant strains were analyzed using Illumina HiSeq 4000 platform and their transcriptome libraries were constructed. After quality filtering, bioinformatics analysis was carried out. The results showed that:A total of 218 significantly different expressed genes (DEGs) between albendazole-susceptible and resistant strains were obtained. Among these 218 genes, 121,82 and 102 DEGs have been annotated to 206 GO terms of three categories respectively, namely biological process, cellular component and molecular function. KEGG analysis showed that 42 significantly DEGs were involved in 31 KEGG pathways, and clustered significantly in pathways of drug metabolism-cytochrome P450, drug metabolism-other enzymes and so on. In addition, we identified 9 drug resistance genes in susceptible and resistant strains, such as GST, CYPs, UGT and so on, which play an important role in the drug resistance of Haemonchus contortus. In this study, the biological characteristics of DEGs in susceptible and resistant strains were predicted by RNA-Seq, which involved functional classification and metabolic pathways. This study provides a foundation for drug target prediction and the establishment of early differential diagnosis methods for drug resistance provides an important basis.

CLC Number:

S855.9

ZHAO Xueliang, WANG Shuyi, SUN Ke, SU Qian, WANG Wenlong, LIU Chunxia. Comparative Transcriptome Analysis of Albendazole-susceptible and Resistant Strains of Haemonchus contortus by RNA-Seq[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1940-1944.

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Comparative Transcriptome Analysis of Albendazole-susceptible and Resistant Strains of Haemonchus contortus by RNA-Seq

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